姓名：杨翌  

研究方向：

研究方向是应用基因编辑技术、诱导多潜能干细胞技术以及单细胞测序技术开展单基因遗传病的干细胞与基因治疗以及疾病动物模型的构建。多年来在基因编辑研究方面取得了多项突破性进展，建立了一系列在生物医药领域具有重要应用价值的动物疾病模型以及基因治疗工具和递送载体的开发和应用，成果包括首次鉴定猪ROSA26位点并建立ROSA26定点敲入Cre重组酶报告基因的大动物模型；建立完全分泌人胰岛素的基因编辑猪，为异种胰岛移植治疗糖尿病提供理想供体；首次建立地中海贫血家兔模型，为地贫临床前基因治疗提供大动物模型；开发Mito-TALEN治疗线粒体遗传疾病的新技术；建立iPSC水平高效无痕、无筛选标记的基因打靶技术体系，为单基因遗传病的干细胞治疗提供新方法；在人造血干细胞水平建立了高效精准的靶向基因编辑体系，开展针对常见β地贫突变位点的通用型原位基因修复方案。目前以第一作者在Cell Research、Protein Cell、JBC等杂志发表高水平SCI论文7篇，作为项目负责人获得国家自然基金青年基金、中国博士后科学基金、广东省、广州市等基金。

代表性论文：

1.Yinghong Yang, Lina He, Yingjun Xie, Lifen Zhu, Jianfeng Wu, Yong Fan, Yi Yang* and Xiaofang Sun*. In situ correction of various β-thalassemia mutations in human hematopoietic stem cells. Frontiers in Cell and Developmental Biology. 2024.1.25

2.Yi Yang^#, Xiangjin Kang^#,Shiqi Hu, Bangzhu Chen, Yingjun Xie, Bing Song, Quanjun Zhang, Han Wu, Zhanhui Ou, Yexing Xian, Yong Fan, Xiaoping Li*, Liangxue Lai*and Xiaofang Sun*.CRISPR/Cas9-mediated β-globin gene knockout in rabbit recapitulates human β-thalassemia. Journal of Biological Chemistry.2021.2.25

3.Yi Yang^#; Han Wu^#; Xiangjin Kang^#; Yanhui Liang; Ting Lan; TianjieLi; Tao Tan; Jiangyun Peng; Quanjun Zhang; Geng An; Yali Liu; Qian Yu; ZhenglaiMa; Ying Lian; Boon Seng Soh; Qingfeng Chen; Ping Liu; Yaoyong Chen; XiaofangSun; Rong Li; Xiumei Zhen; Ping Liu; Yang Yu^*; Xiaoping Li^*; YongFan^*,Targeted elimination of mutant mitochondrial DNA in MELAS-iPSCs by mitoTALENs. Protein & Cell, 2018.1.9

4.Yali Liu^#,Yi Yang^#, Xiangjin Kang^#, Bin Lin, Qian Yu, Bing Song, Gao Ge, Yaoyong Chen, Xiaofang Sun, Xiaoping Li^*, Lei Bu^*, and Yong Fan^*. One-step biallelic and scarless correction of a β-thalassemia mutation in patient-specific iPSCs without drug selection. [J]. Molecular Therapy：Nucleic Acids, 2017.3.6

5.Yi Yang^#, Kepin Wang^#, Han Wu, Qin Jin, Degong Ruan, Zhen Fan, Qishuai Liu, ShimeiGuo,Lei Bu, Yong Fan, Xiaofang Sun,Xiaoping Li^*,Liangxue Lai^*. Genetically humanized pigs exclusively expressing human insulin through custom endonucleases mediated seamless engineering [J]. Journal of Molecular Cell Biology, 2016. 8

6.Xiaoping Li^#,Yi Yang^#, Lei Bu, Xiaogang Guo, Chengcheng Tang, Jun Song, Nana Fan, Bentian Zhao, Zhen Ouyang, Zhaoming Liu, Yu Zhao, Xiaoling Yi, Longquan Quan, Songcai Liu, Zhenguo Yang, Hongsheng Ouyang, Y Eugene Chen, Zhong Wang^*, Liangxue Lai^*. Rosa26-targeted swine models for stable gene over-expression and Cre-mediated lineage tracing[J]. Cell research, 2014. 2.24

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